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In cultured human breast cancer cells containing estrogen receptors, estradiol stimulated growth when added at concentrations of 10 -12 mol/L and above, with a maximal response at about 10 -10 mol/L

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As we discuss in this section and in section III.C.2.c.(1) of this final rule, we believe mandatory participation is necessary to test ASM due to concerns that selection bias in a voluntary model would undermine ASM's incentives